BTC Collaborating with Globally Recognized Biotech Organizations
Brimrose Technology (BTC) is working with a variety of globally recognized institutes and companies in helping us get this division launched, including the Eliava Institute in Tbilisi, Georgia; the Jiangsu Academy of Agricultural Sciences, in Nanjing, China; NHDetect, Baltimore; and the International Phage Research Center, China.
BTC already is involved in several critical research operations that we believe will be beneficial.
One focus of research is on the utilization of the bacteriophage virus and Phage Lytic Enzymes for the treatment, prevention, control and detection of specific bacteria in the environment or in applications for agriculture, animal or food use. Bacteriophages, or bacteria eaters, occur in nature, kill bad bacteria, are safe for animals, are inexpensive, and do not harm the environment or cause the problems that antibiotics do. While bacteriophages have been used for some time with varying degrees of success, work by Trudil, the aforementioned Georgian and Chinese institutes, and other researchers working with Brimrose show significant progress and should lead to greater use of these important viruses.
Working with the Eliava Institute, our new Biotechnology Division is offering a series of unique bacteriophage products, which are being used to replace antibiotics in feed, environmental control of bacteria, rapid detection and for other important applications. As mentioned, bacteriophages are “good” viruses that attack and kill bad bacteria that can harm poultry and other living things. (Click on the bacteriophage product offerings
we are introducing.)
The new division also is working with partners on the development of a Membrane Enhanced Fluorescence Detection System with specific monoclonal antibodies for the rapid and sensitive detection of bacteria, viruses and toxins based on immunological (antibody-antigen) reactions. A combined 180 degree optical/membrane configuration will be employed for rapid, high sensitivity and simple determination of low concentrations of target substances such as bacteria, spores and viruses.
The fluorescence antibody mixture is immobilized on a carefully selected translucent membrane with appropriately low fluorescence background and high sensitivity characteristics.
In a new advancement to Hand Held Assays, the polymer enhanced SMART lateral flow assay provides an increase of up to 1 log in sensitivity as part of the first bio-defense test used since Desert Storm I in 1991. This new assay can be used for the detection of unknown bacteria, viruses and toxins for defense, food, animal or environmental applications.
We also are working collaboratively in the area of Bioluminescence - Detection of ATP from bacterial cells. The first assay is related to a generic bacterial detection system to determine general contamination. Through these efforts, we can identify specific bacteria with the inclusion of specific phage lytic enzymes (gram positive lytic enzyme detection).
The bioluminometer shown here is able to determine the presence of low levels of bacteria and spores. The system generally provides results within five minutes for bacterial detection and within 20 minutes for spore detection.